Scarlette is Bobbery's deceased wife in Paper Mario: The Thousand-Year Door. She is never seen in the game. As the player learns from Podley, during one of Bobbery's sailing trips, she succumbed to a fatal illness. He did not know if it was a cold or a virus. But it killed her. Bobbery had no clue of his wife's condition. By the time he had returned, she was already dead. Bobbery blamed himself for her death because he thought that if he had not been on a trip at the time, he could have nursed her to health. He resolved to never sail again, until Mario gave him a letter written by Scarlette that had been held by Podley for many years. Podley did not have the heart to give him the letter, seeing his grief. He thought it best it be put behind him. In her last letter, she tells Bobbery not to blame himself for her death and to keep doing what he loves: sailing the seas. After reading the letter, Bobbery says, "Yes love... I WAS happy... My sweet, sweet Scarlett, I love you still."
The only other time she is ever mentioned is at the end of the game. When the Shadow Queen demanded for Mario and partners to become her servants, if Bobbery is the current partner, he says, "I think Scarlette would be angry if we don't refuse." Also, at the very end, when Peach is unpossessed, he says, "By Scarlette's soul! Look, Mario!". When Goombella says Bobbery is now sailing the seven seas with Cortez, she says Scarlette is watching over him.