In the Piranha Plant Tour, Piranha Plant Slide served as the tour's signature course. It appeared as the first course in the Dry Bones Cup, the third course in the Donkey Kong Cup, and the location of the bonus challenges in the Toadette Cup and the Dry Bowser Cup. Its T variant appeared as the second course in the Ice Mario Cup, the first course in the Daisy Cup, and the location of the bonus challenge in the Mii Cup, and its R variant appeared as the second course in the Mario Cup and the third course in the Toad Cup.
In the 2022 Bowser Tour, Piranha Plant Slide appeared as the second course in the Mii Cup. Its R variant appeared as the first course in the Nabbit Cup, and its T variant appeared as the second course in the Kamek Cup.
In the 2022 Mario vs. Luigi Tour, Piranha Plant Slide appeared as the first course in the Pauline Cup. Its T variant appeared as the third course in the Mii Cup, and its R variant appeared as the first course in the Toad Cup.
In the Animal Tour, Piranha Plant Slide appeared as the first course in the Pauline Cup. Its T variant appeared as the second course in the Kamek Cup and the location of the bonus challenge in the Roy Cup, and its R variant appeared as the third course in the Dry Bowser Cup.
In the 2023 Exploration Tour, Piranha Plant Slide appeared as the third course in the Fire Rosalina Cup and the location of the bonus challenge in the Pink Gold Peach Cup. Its R variant appeared as the first course in the Monty Mole Cup, its R/T variant appeared as the second course in the Yoshi Cup, and its T variant appeared as the third course in the Ice Bro Cup.
In the Pipe Tour, Piranha Plant Slide appeared as the first course in the Dixie Kong Cup and the location of the bonus challenge in the Donkey Kong Cup. Its T variant appeared as the third course in the Tanooki Mario Cup, its R variant appeared as the third course in the Toad Cup, and its R/T variant appeared as the second course in the Fire Rosalina Cup.